Then CHARACTERISATION From One or two ARTEMIA Populations Off Northern GREECE: BIOMETRY, HATCHING Attributes, CALORIC Posts And you can Greasy Acid Profiles

Embolon, Thessaloniki, was in fact classified on their form of reproduction and you can ploidy

BOWEN, S.T., DURKIN, J.P., STERLING, G. and you can CLARK, L.S. (1978) Artemia hemoglobins: hereditary variation into the parthenogenetic and you will zygogenetic populations. Bull., 155, 273287.

Cysts of parthenogenetic Artemia stresses amassed from the Citros and you will M. Embolon saltworks when you look at the North Greece is examined due to their prospective explore into the aquaculture. Another characterizations was indeed performed: tumefaction and you can naupliar biometrics, tumor hatching services, oily acidic reputation of the nauplii, caloric content away from nauplii kept during the 25° C as well as in a fridge (cuatro 8° C). The above evaluation suggests that both Artemia strains analyzed exhibit a beneficial qualities to be used inside aquaculture, particularly in culturing new-water types. New biometrical research off cysts, nauplii and people suggests a leading standard of similarity along with other parthenogenetic strains out of some geographical provide, but especially which have tetraploid Artemia off The country of spain. The fresh new Greek Artemia challenges can not be considered as ‘supply to have aquacultural spends except if right handling of the brand new saltworks are in hopes.

Once the brine shrimp Artemia try of highest financial importance, you will need to analyse the fresh new ‘commercial qualities of numerous populations pertaining to its prospect of software for the aquaculture. In earlier degree, several Northern Greek Artemia communities found in Citros, Piera and M. These people were discovered to be parthenogenetic and you may tetraploid (Abatzopoulos et al., 1986). Analysis out of degree for the DNA reassociation Kinetics and on thirteen enzymatic expertise of the two communities imply that it fall into one to kinds (Abatzopoulos et al., 1987).

Biol

Inside paper i report about the fresh biometrical services of cysts, nauplii and you may grownups, towards the hatching services out-of cysts, i.elizabeth. hatching rate, hatching fee, hatching show and hatching returns, to your changes in deceased lbs and caloric stuff out of instar-I nauplii was able at room temperature and cold-storage conditions, as well as on the new greasy acidic users away from instar-We nauplii.

The two populations from Northern Greek Artemia evaluated come from the fresh new saltworks out-of Citros, Pieria (C strain), and from the saltworks from M. Embolon, Thessaloniki (Me personally filters). Cysts had been compiled inside the 1983, processed according to Sorgeloos et al. (1978), and you may stored in shut plastic handbags in the -25° C, having future use.

Mature brine shrimps was anesthezied during the chlorofrom-saturated and you will mentioned below a dissection microscope playing with a great Leitz micrometer, Another measurements have been performed : total size, intestinal duration, maximum thickness off brood pocket, width out of third abdomianal portion, thickness out-of lead, length of earliest antenna, maximum diameter of complex eyes, and point between advanced attention (Amat, 1980). Proportions had been did into animals – attempt size = fifty pets-from nuts cysts and cultured lower than simple labouratory requirements (fungus given that dinner, temperatures regarding 25 + 1° C, and you may seawater away from thirty-five% salinity). The details was in fact mathematically managed by investigation out of covariance (Sokal Rohlf, 1981).

Hatching analyses was indeed accomplished according to Vanhaecke Sorgeloos (1983). Hatching overall performance, hatching percentage, hatching speed and hatching output was analysed following speed and hatching returns was indeed analysed pursuing the Sorgeloos mais aussi al. (1978), Bruggeman et al. (1980), Vanhaecke Sorgeloos (1982), and you can Vanhaecke Sorgeloos (1983), correspondingly.

The brand new hatching of one’s cysts additionally the separation and line of the latest nauplii (instar-l to instar-III) was indeed carried out with regards to the process revealed when you look at the Vanhaecke e al. (1983).

The fresh new types of nauplii was indeed dried in an incubator on 55°C to have forty eight hrs. For every single naupliar stage examined, four to six duplicated samples, of approximately 10 milligrams dead point had been burned totally inside pure clean air as well as their caloric content was measured in the an effective Phillipson microbomb calorimeter.

Just before study, samples was basically homogenized in the an enthusiastic ultrasonic homogenizer (Sonifier B12) and overall lipids were removed depending on the type of Bilgh Dyer (1959). Saponification and you will methylation had been did as the discussed by the Shauer Simpson (1978). Oily acid methylesters was indeed injected inside an effective capillary column (twenty-five m bonded silica; interior diameter; 0.32 mm; liquide phase : SILAR 10C, flick thickness: 0.3um) installed when you look at the good Carlo Erba Super 2350 energy chromatograph.